2013  March  7
Protein level analysis by ELISA

Unique ID


QPCR shows induction of the mRNA by the pharmacological inducing agent.
Here, protein levels are measured.


This experiment measures the total concentration of protein expressed by the cells in the
cell culture by antibody-conjugated reporter system. First, cells are grown and treated with the
pharmacological inducing agent. Second, a commercial ELISA kit is used.

  A brief comment  |  Thu Mar 7 16:30:53 EST 2013
Total protein concentration measurement agrees with the QPCR analysis.
The data from QPCR are shown below both as an Xcel file and a screen shot.

# Human cells

Plate image